Part:BBa_K277053

3L.3_23.B3.01

3L.3_23.B3.01 is 751 bases long and is cloned into the pGem-T vector.

3L.3_23.B3.01 was designed as a piece of synthetic chromosome 3 with the goal of minimizing and stabilizing that chromosome and to that end has had any tRNAs, introns, repeat regions, and transposons that were present in the wildtype chromosome removed. In addition a very few gene sequences were slightly recoded to add or remove restriction enzyme recognition sites to facilitate assembly; most gene sequences were slightly recoded to introduce unique primers for diagnostic PCR amplification, and some gene sequences were slightly recoded to address the distribution of stop codon usage. 3L.3_23.B3.01 is a constituent of 3L.3_23.B3 (along with 3L.3_23.B3.02, 3L.3_23.B3.03, 3L.3_23.B3.04, 3L.3_23.B3.05, 3L.3_23.B3.06, 3L.3_23.B3.07, 3L.3_23.B3.08, and 3L.3_23.B3.09.)

This part contains at least part of the following features (positions offset from first base of sequence):

kind and name offset notes

gene YCL043C (231..+1799) Protein disulfide isomerase%2C multifunctional protein resident in the endoplasmic reticulum lumen%2C essential for the formation of disulfide bonds in secretory and cell-surface proteins%2C unscrambles non-native disulfide bonds

reverse_primer YCL043C_tagr1v1 (705..732)

forward_primer YCL043C_tagf1v1 (375..402)

mutation_affecting_coding_sequence YCL043C_re_remove_Bpu10I (288..296) removal of Bpu10I

mutation_affecting_coding_sequence YCL043C_re_remove_MmeI (513..524) removal of MmeI

Sequence (the first 751 bases correspond to coordinates 38762..39512 in synthetic chromosome yeast_chr3_3_23

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 714
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 689
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]